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Plasma nicotine was analysed by liquid chromatography-tandem mass spectrometry (LCMS/MS) at Celerion Bioanalytical Services (Lincoln, Nebraska, USA) using a validated analytical method with appropriate quality controls according to the Food and Drug Administration (FDA) Guidance for Industry (Title 21 Code of Federal Regulations Part 58)

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"Detection of Cotinine in Blood Plasma by HPLC MS/MS"